TAYROID STIMULATING HORMONE EXTRACTED FROM HUMAN PLACENTA

[Purification of follicle-stimulating hormone from human pituitary glands].

A highly purified follicle-stimulating hormone from human pituitary glands has been prepared by ammonium sulfate and ethanol fractionation, gel filtration on Sephadex G-100, ion exchange chromatography on carboxymethyl cellulose, zone electrophoresis on cellulose columns, and preparative continuous flow polyacrylamide gel electrophoresis. The final product has shown lO,OOO-fold purification ove...

Purification of Follicle-stimulating Hormone from Human Pituitary Glands*

A highly purified follicle-stimulating hormone from human pituitary glands has been prepared by ammonium sulfate and ethanol fractionation, gel filtration on Sephadex G-100, ion exchange chromatography on carboxymethyl cellulose, zone electrophoresis on cellulose columns, and preparative continuous flow polyacrylamide gel electrophoresis. The final product has shown lO,OOO-fold purification ove...

Thyroid Stimulating Hormone Thyroid Stimulating Hormone

Follicle-stimulating hormone and human spermatogenesis.

The role of follicle-stimulating hormone (FSH) in the control of spermatogenesis is not well established in any species, including man. We studied the effect of an experimentally-induced, selective FSH deficiency on sperm production in normal men. After a 3-mo control period, five normal men received testosterone enanthate (T) 200 mg i. m. weekly to suppress luteinizing hormone (LH) and FSH, un...

Overnight radioimmunoassay of human follicle-stimulating hormone.

A radioimmunoassay procedure for follicle-stimulating hormone in serum has been developed in which the assay mixture is incubated at 37#{176}C for a total of 22 h, rather than at 4#{176}C for 4-7 days. The withinassay coefficients of variation for the 37#{176}C and 4#{176}C methods, respectively, are 7.7% and 5.0%, while the between-assay variations are 13.8% and 9.9%. Comparison of values for ...